Research Articles
Advancing CDG Diagnosis: A Comprehensive Guide to Serum N-Glycome Analysis Using HILIC-UPLC-ESI-MS
Congenital Disorders of Glycosylation (CDGs) represent a complex group of metabolic diseases with significant diagnostic challenges.
A Complete HILIC-UPLC Workflow for Fluorescently Labelled Glycan Analysis: From Release to Data Interpretation
This article provides a comprehensive guide to the complete HILIC-UPLC workflow for the analysis of released and fluorescently labelled glycans, targeted at researchers and biopharmaceutical professionals.
Fc-Glycosylation Analysis Decoded: Comparing HILIC-UPLC and Mass Spectrometry for Therapeutic Antibody Profiling
This comprehensive guide for researchers and biopharmaceutical professionals explores the critical methodologies for Fc-glycosylation profiling of therapeutic antibodies.
HILIC-UPLC vs Capillary Electrophoresis: A Precision Showdown for Glycan Analysis in Biopharmaceuticals
This comprehensive analysis compares the precision, throughput, and application scope of Hydrophilic Interaction Liquid Chromatography (HILIC-UPLC) and Capillary Electrophoresis (CE) for glycan analysis, a critical step in biopharmaceutical development.
HILIC-UPLC vs. MALDI-TOF-MS: A Comprehensive 2024 Guide for IgG Glycan Profiling in Biopharma
This article provides a detailed, comparative analysis of Hydrophilic Interaction Liquid Chromatography coupled with Ultra-Performance Liquid Chromatography (HILIC-UPLC) and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) for the analysis of...
HILIC-UPLC vs. CGE-LIF for High-Throughput Glycomics: A 2024 Guide for Researchers
High-throughput glycomics is essential for biomarker discovery and biopharmaceutical development, requiring robust analytical platforms.
HILIC-UPLC vs. CE-LIF for Glycan Analysis: A 2024 Guide to Accuracy, Speed, and Choice for Biopharma
This comprehensive guide analyzes two leading techniques for N-glycan profiling in biopharmaceutical development: Hydrophilic Interaction Liquid Chromatography-Ultra Performance Liquid Chromatography (HILIC-UPLC) and Capillary Electrophoresis with Laser-Induced Fluorescence (CE-LIF).
Optimized HILIC-UPLC Protocol for IgG N-Glycan Analysis from Plasma: A Step-by-Step Guide for Biomarker Research
This article provides a comprehensive guide for researchers and biopharma professionals on implementing a robust HILIC-UPLC method for analyzing IgG N-glycans from plasma.
Mastering HILIC-UPLC for Biopharmaceutical Analysis: A Comprehensive Guide to Glycoprotein Profiling and Therapeutic Development
This article provides a comprehensive overview of Hydrophilic Interaction Liquid Chromatography coupled with Ultra-Performance Liquid Chromatography (HILIC-UPLC) for the analysis of biopharmaceuticals and glycoprotein therapeutics.
HILIC-UPLC for Glycan Separation: Principles, Methods, and Best Practices for Biopharmaceutical Analysis
This comprehensive article explores Hydrophilic Interaction Liquid Chromatography coupled with Ultra-Performance Liquid Chromatography (HILIC-UPLC) as a critical analytical technique for glycan separation.